Device
Part:BBa_K176065:Design
Designed by: Zongxiao He, Hao Jiang Group: iGEM09_USTC (2009-07-30)
aTc&AHL->PoPS: pCon 0.70->tetR-LVA+pCon 0.70->luxR+pLux/Tet->PoPS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 898
Illegal NheI site found at 921 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1876
Design Notes
Input1: aTc
Input2: AHL
Output: PoPS
Design:
pCon 0.70(J23101) generates TetR-LVA
pCon 0.70(J23101) generates LuxR
pLux/Tet(K176000) generates PoPS, activated by LuxR and Input2 AHL, repressed by TetR-LVA and no Input1 aTc
Source
J23101 K176032